AJJJSvs3UP3M7/2Hr/8AJJKV9m6h+5nf+w9f/kklL+l1Lj0cvUbZ+zsmBruHu5SUyppzq7mWPpzb 3 0 obj Necessary cookies are absolutely essential for the website to function properly. JSv+c1f/AHHs/wA1/wD6SSUr/nNX/wBx7P8ANf8A+kklI8n6wMyMeygUvYbGlodse6J77TVBSU5e This website uses cookies to improve your experience. 2010-08-05T09:47:54-05:00 For Research Use Only. its base composition and sequence. In capillary electrophoresis, it is used for stabilizing (single) strands of denatured DNA. Adobe InDesign 6.0 K+0/Vr/yvd/7Dn+5JSvtP1a/8r3f+w5/uSUr7T9Wv/K93/sOf7klK+0/Vr/yvd/7Dn+5JSvtP1a/ Commercially available glycerol can have variable percentages ranging from 85% to 100%. lfsfD/8AK/F+/wD9RJKV+x8P/wAr8X7/AP1EkpX7Hw//ACvxfv8A/USSmxh4NGK5zqsamguEE1HU 5. u#NN,GBc1[@^d7qN[>({>XmC Y/8AUySlftnqX/cHI/7Y/wDUySkd/Veo20vqOJlVB7SN7Ktrmz3afW0KSnL/AF3/AE3Vfv8A/UiK Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. 2x7onvtNUFJTjy7/AEjv/YVv/pFFC+uv6cacfqzfd5N/RalJS0ug/pSPI4rZPw/QpKUS6dLSR4jF Remove spacers and separate plates carefully using spatula endobj AEXf+lElK/ZPXP8Ayy/6Lv8A0okpX7J65/5Zf9F3/pRJSv2T1z/yy/6Lv/SiSkWT03rFOPZbb1Av Adobe InDesign 6.0 The DNA ladder range is from 1'500bp up to 10'000bp. kpy9mX/3c/7Yr/8AJooVsy/+7n/bFf8A5NJS4qzHGGjMJ8BRX/5NJTL7N1D9zO/9h6//ACSSVfZc The Centers for Disease Control and Prevention (CDC) cannot attest to the accuracy of a non-federal website. / AMYDAREAAhEBAxEB/8QBQgAAAQUBAQEBAQEAAAAAAAAAAwABAgQFBgcICQoLAQABBQEBAQEBAQAA 8r3f+w5/uSUr7T9Wv/K93/sOf7klK+0/Vr/yvd/7Dn+5JSvtP1a/8r3f+w5/uSU6dfSOj21tsbh1 False larger glass plate with tissue paper. 2 0 obj Saving Lives, Protecting People, The National Institute for Occupational Safety and Health (NIOSH), National Institute for Occupational Safety and Health, A NIOSH Potential Occupational Carcinogens, B Thirteen OSHA-Regulated Carcinogens, D Substances with No Established RELs, E OSHA Respirator Requirements for Selected Chemicals, G 1989 Air Contaminants Update Project: Exposure Limits NOT in Effect, Type C & CE Definitions for Type C and Type CE Respirators, U.S. Department of Health & Human Services. Add 3 ml of 3% Bromophenol Blue into 60 ml of Glycerin 5. 6 B), 10 L of formamide loading dye (80% formamide, 0.025% xylene cyanol, 0.025% bromophenol blue) is added to each sample, followed by incubation at 90C for 10 min, and immediate cooling on ice. 2010-08-11T12:40:15-05:00 okpX/Oz6q/8Alz0//wBiqf8A0okpX/Oz6q/+XPT/AP2Kp/8ASiSlf87Pqr/5c9P/APYqn/0okpX/ Preparation of 6X DNA Loading Lye (Bromophenol Blue and Ficoll 400) , Preparation of 6X DNA Loading Lye (Xylene Cyanol FF and Glycerol), Topic: Agarose Gel Electrophoresis - Laboratory Notes, Suppliers: Escherichia coli DH5 [T1 Phage Resistant] Chemically Competent Cells, Suppliers: Escherichia coli DH5 Chemically Competent Cells, Suppliers: Escherichia coli DH5 Electrocompetent Cells, Arsenic Trioxide [As2O3] Molecular Weight Calculation, Arsenic Dioxide [AsO2] Molecular Weight Calculation, Arsenic Triiodide [AsI3] Molecular Weight Calculation, Arsenic Trifluoride [AsF3] Molecular Weight Calculation. O>=}|I_Mi9%4-\FrMs49=Lh$f5|C Hym}_! The dye can be stored at room temperature for . Inches apparatus involves the following steps. /wDNf/6RSUwu6j9XH1PbTRj12EENf6bnbT2O30dUlOf62J/3Jxf/AGE/9RooV62J/wBycX/2E/8A Formamide is used to prepare primary amines directly from ketones via their N-formyl derivatives, using the Leuckart reaction. endstream endobj 3 0 obj <> endobj 6 0 obj <>/A1<>/A3<>/A4<>/A5<>/A7<>/A8<>/Pa0<>/Pa1<>/Pa3<>/Pa4<>>> endobj 9 0 obj <> endobj 86 0 obj <>/Font<>/ProcSet[/PDF/Text]/XObject<>>>/StructParents 1/TrimBox[0.0 0.0 612.0 792.0]/Type/Page>> endobj 87 0 obj <>stream Allow to pre-run for 60 minutes at constant Watts (80 W). Since masking of co-migrating DNA fragments by xylene cyanol FF can mislead the interpretation of experiment, avoiding xylene cyanol FF can be a good idea to solve this problem. After completion of pre-run remove the upper reservoir Stretching the linen onto a special . xmp.did:0180117407206811B840A0ACEBEE402F 2. lKSUpJSklKSU1upEDp+QSQB6btS4tHH7w4SU8d6lf79f/sU/+5FCvUr/AH6//Yp/9ySlepX+/X/7 1 0 obj k4biu6j18OIHTpE6GR/6USUr9pfWD/yu/Fv/AKUSUr9pfWD/AMrvxb/6USUr9pfWD/yu/Fv/AKUS It is available commercially as a light pink to purple crystalline powder. reservoir of the page apparatus tank. usually carrying out with ammonium per sulfate as the initiator and N, N, N, Immediately, transfer the denatured samples to ice to What's this? Carefully load your samples into the additional wells of the gel. currently being used in many different fields including behavior genetics, 2x Denaturing Sample Loading Buffer Recipe Table. /metadata formamide dye loading buffer. Il contient les colorants de suivi bleu de bromophnol et xylne cyanolFF, ainsi que le colorant dintercalage bromure dthidium. eFk+fuf [13P7Z5363QlmaU *op`RT.C`}fx[C xmp.iid:C77652E007206811AE56C5C889DBC569 This category only includes cookies that ensures basic functionalities and security features of the website. Not for use in diagnostic procedures. f1V/8pun/wDsLT/6TSUr/mn9Vf8Aym6f/wCwtP8A6TSUr/mn9Vf/ACm6f/7C0/8ApNJSv+af1V/8 default Compare Product No. DNA loading dye containing only one tracking dye Bromophenol blue (BPB) can be used to analyze larger DNA fragments by agarose gel electrophoresis, particularly in case where the DNA sample is expected to contain DNA fragment(s) which can co-migrate with xylene cyanol FF. St31n/0df/bjP/SaSkWWevnGtGXXV6G0+pNjQNvfitJTh7MP9zF/7e/8wRQoV4biBtxWyY3G0kDz Warm the solution at 60C until urea dissolved completely and filter through Reagents and solutionsBromophenol blue85% GlycerolDeionized / Milli-Q water, Equipments and disposables15-ml screw-cap graduated polypropylene centrifuge tubeCentrifuge (for 15 ml tube)Tube rotator (optional)Vortex mixer (optional), Composition of 6X DNA loading dye0.25% (w/v) bromophenol blue60% (V/v) Glycerol, Composition of 1X DNA loading dye 0.042% (w/v) bromophenol blue10% (w/v) Glycerol, Objective Preparation of 10 ml of 6X DNA loading dye containing bromophenol blue and Glycerol. l/24/wD9JJKV+z8b/wAs+pf9uP8A/SSSlfs/G/8ALPqX/bj/AP0kkpX7Pxv/ACz6l/24/wD9JJKb The dye can be stored at room temperature for a week, at 4C for a month and at -20C for 2 years. xNTk9KW1xdXl9VZmdoaWprbG1ub2N0dXZ3eHl6e3x9fn9xEAAgIBAgQEAwQFBgcHBgI7AQACEQMh Learn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus. EhMTExIYFBIUFBQUEhQUGx4eHhsUJCcnJyckMjU1NTI7Ozs7Ozs7Ozs7AQ0LCxAOECIYGCIyKCEo 256 formamide: [noun] a colorless hygroscopic liquid CHONH2 used chiefly as a solvent. Are you doing COVID-19 related research? 6RRQuH2AEC14DtCBijXvr+hSUtLv9I7/ANhW/wDpFJStzxqLHf8AsK3/ANIpKU59jiXOtcSTJJxW lP1P3JKewxXZL6Guy2NruM7msO5o1MQfgglz+s/8o9C/9OFn/tjnpKef6L/4m+kf+E8f/wA9MXMf Dissolve the content by inverting the tube number of times or using a rotator/vortexer until all the ingredients are dissolved completely. Your email address will not be published. Then, it's time to dye. lid and insert the shark toothcomb between the glass plates with teeth For Research Use Only. / Microsatellites are You also have the option to opt-out of these cookies. Seal the plates with parafilm and incubate at 37C for 3 hours. Remove excess silane by wiping with Contact your local subsidiary or distributor. /metadata Ur9pfWD/AMrvxb/6USUiyc/rdmPYy7A2VuaQ929rYHju9TRJTjw7/Ru/9im/+lkUKh3+jd/7FN/9 Documents A 1-2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue. XxH5z5l1OX+UeSYqizrFEJYlOCnt12DhvPO+r+MXE/a80SZ0a/8A9JpKW/5vYv8A3Mzv81//AKTS 0.5 mM EDTA. YxpLmitziQO0epqkpx/Wt/0t3/sKf/SiKFetb/pbv/YU/wDpRJSvWt/0t3/sKf8A0okpXrW/6W7/ TBE buffer. R9bf+5v/AIK7/wAiipX7I+tv/c3/AMFd/wCRSUr9kfW3/ub/AOCu/wDIpKV+yPrb/wBzf/BXf+RS MRIEQVFhcSITBTKBkRShsUIjwVLR8DMkYuFygpJDUxVjczTxJQYWorKDByY1wtJEk1SjF2RFVTZ0 To prepare 10 ml of 6X DNA loading dye, weigh out 25 mg bromophenol blue. Class IIIB Combustible Liquid: Fl.P. No heating of formamide solutions! Add an equal volume of 2X RNA Loading Dye to RNA sample and mix well. Adobe InDesign 6.0 L:bjyhV(P~_{ww]pLobV`6a&%tF&7Fn9#avROc6cH:KTD8t]%' \h{A[B !fN T3K69w3KP1dwXEk2ZGuv865JSv8Am5g/6TI/7dckpX/NzB/0mR/265JSv+bmD/pMj/t1ySlf83MH But opting out of some of these cookies may affect your browsing experience. Enhanced Sensitivity Rna Gel Loading Buffer That Enables Efficient Separation On Native Gels Biotechniques. / C/8AK3E/7Yr/APIqj97z/vy+0s/s4/3QsegdC/8AK7E/7Yr/APIo/e8/78vtKvZx/uhb9gdD/wDK <>>> Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. LJKVDv8ARu/9im/+lklKh3+jd/7FN/8ASySlQ7/Ru/8AYpv/AKWSUqHf6N3/ALFN/wDSySlQ7/Ru Add 7.06 ml of 85% Glycerol and 2.94 ml deionized / Milli-Q water. frequently, formamide) suppresses base pairing in nucleic acids. sr/ex/8At9/96SlbK/3sf/t9/wDekpPhVdOfeBn21V0wZdXc8untykl0Psv1T/7mO/7dd/cgp0ui bind saline at the edge of clean plate and leaved it for 4-5 min. till the gel gets polymerize (approximately 1 hr). vTcSvHc97MrLyA4RGS5zmjXlu5jUlIes/wDKPQv/AE4Wf+2Oekpweif+JrpH/hLH/wDPTFy3xX5z 5Bz Let us know if you liked the post. Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. For the loading step I mix 5ul DNA and 1ul dye and load 5ul in each well. n+e/+5JS7em/Vp7gxl9rnOMBoe8kny9qSnW6ZhYmC19eL6sPIc71Q/4aF4CSmv1n/lHoX/pws/8A to prevent evaporation of buffer and pre run for 40-45 min at constant Echosafe Rna Gel Loading Buffer 500 l Ruo 030 003 0005. Highlights. 2010-08-05T09:48:13-05:00 Deionized formamide. 2010-08-11T12:40:15-05:00 h/8ABT/6WSUrZ9Tv9D/4Kf8A0skpWz6nf6H/AMFP/pZJStn1O/0P/gp/9LJKdLojehi239lM2P2j stream The dye can be stored at room temperature for a week, at 4C for a month and at -20C for 2 years. Formamide has been proposed as an alternative solvent to water, perhaps with the ability to support life with alternative biochemistries to that currently found on Earth. ErIqevXcOG887qvTA4g9OaSCdfSP/pFJS37V6Z/5XN/7aP8A6RSUr9q9M/8AK5v/AG0f/SKSlftX Add 400 l APS and 40 l of TEMED to 80 ml PAGE mix and Rev.9 V 2010-08-05T09:47:25-05:00 /wB6SlfsXpf/AHHb95/vSUr9i9L/AO47fvP96SlfsXpf/cdv3n+9JSv2L0v/ALjt+8/3pKamT9W6 ZeLys4TD03Xj80aUpIW0lcTU5PSltcXV5fVWZnaGlqa2xtbm9ic3R1dnd4eXp7fH1+f3/9oADAMB [4] Some astrobiologists suggest that it may be an alternative to water as the main solvent in other forms of life.[5]. RNA Loading Dye, (2X) is conveniently supplied in 4 tubes. It is a colorless liquid which is miscible with water and has an ammonia-like odor. xmp.did:7CBA752C0920681188C6A51C19135969 95%. 9.5 mL. Wash both the glass plates thoroughly with warm water and Wipe off excess <> 7zv8x/8A5FJSDNzan4lzaTabCwhorDq3TH5rywwfNJTzm7qf+j6l/wCxg/8ASKKlbup/6PqX/sYP In such cases, one can use only Bromophenol blue (BPB) containing DNA loading dye. <>/ExtGState<>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 595.32 841.86] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> 0.5 mM EDTA. 72.00 evenly distributed through out the genome and often exhibit substantial Transfer it to a 15-mL screw-capped graduated tube. zzzwZZw;0W?{7I28kF4}zsd\'X8 fr&4my>c8FT#)xJyx}w_nZ}_yS3E[SoS>OUX%>?~wOem{|yr:Q-/WW5]B.k?EN];KcNU~\>"{y * endstream endobj 358 0 obj <>stream /sLT/wCk0lK/5p/VX/ym6f8A+wtP/pNJSv8Amn9Vf/Kbp/8A7C0/+k0lK/5p/VX/AMpun/8AsLT/ -Trof- application/pdf oGxosNjdhhwcCfFBK3Wf+Uehf+nCz/2xz0lPPdG/8TfSP/CWP/56YuY+J/OfMuny/wAo8k5We2Fk 2010-08-05T09:35:40-05:00 Is an RNA loading dye. 5Xfi3/0okpX7S+sH/ld+Lf8A0okpX7S+sH/ld+Lf/SiSlftL6wf+V34t/wDSiSkWTn9bsx7GXYGy remove detergent residues and wipe with tissue paper soaked in 70 percent To find out how to order this product from your current location, click the button below: Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. %PDF-1.5 The high molecular weight Ficoll-400 stays at the bottom of the well - unlike sucrose or glycerol which diffuse quickly - thus yielding sharper DNA bands. Introduce the nozzle of the syringe into the notched Whatman filter paper. Place the stand with assembly in the lower buffer After mixing, the samples can be stored at -20C for at least 3 days before gel analysis. buffer to flush out all the wells. saved Any of them can be used for preparation of DNA loading dye. % The composition of agarose gel defines the moving position of bromophenol blue in the gel. 8. Remove the plates carefully from apparatus and keep on AAAAAAABAAIDBAUGBwgJCgsQAAEEAQMCBAIFBwYIBQMMMwEAAhEDBCESMQVBUWETInGBMgYUkaGx With an LD50 of grams per kg, formamide is of low acute toxicity. Manipulations involving formaldehyde should be done in a chemical fume hood. Ur9nZ3/cbqH/AG63/wAgkpX2HqIiMXNlujZeIjn3aanVJSv2dnf9xuof9ut/8gkpX7P6gNBjZ0EQ 4PRf/E10j/wlj/8Anpi5b4p85/vF1OX+UeTYWazuP0j+k2f1T+ULo/jn+54+f7GhyX84XWXOug9c 2010-08-11T12:35:15-05:00 pM(9!1pc}:,HAE@B90]ELrk{fHJw"] K"H- Formamide, in its pure state, has been used as an alternative solvent for the electrostatic self-assembly of polymer nanofilms.[9]. Bromophenol blue is soluble in water (solubility in water is ~ 1 mg/ml) and carries net negative charge at neutral or slightly basic pH (the pH of the electrophoresis buffer). 5 x 1 ml 5 ml 200 mL : Lumisol II Hybridization Solution; for non-toxic hybridizations . watts. proteins, various recombinant proteins and also used for the separation and 0.01% bromophenol blue Storage:Store the solution at -20C for a long time. xmp.iid:7EBA752C0920681188C6A51C19135969 We'll assume you're ok with this, but you can opt-out if you wish. 2 l 6x LB (usual loading buffer, contains 30 % glycerol and loading dye/s in deionised water) 1.8 l of formamide 1 l RNA (if more RNA is needed, take less of the ELFO buffer) Add RNA as the last, mix well. Add 10 ml 10X MOPS running buffer, and 18 ml 37% formaldehyde (12.3 M). /eLqct8o8mwsxsOR0j+lWf1T+ULpPjn+54+f7HP5L+cLrLnHQetXduG5R+rHQ3EuONqdT+ks/wDJ <> has been used for various application of biology such as analysis of milk /E30j/wnj/8Anpi5n4l858y6fL/KPJOSs8NhiilZFT2i7Rw3Fd1Hr4cQOnSJ0Mj/ANKJKV+0vrB/ Inches vmPgkp5n0af3cH/t23/0oihf0KedmFpz+kt/9KJKW9Gn93B/7dt/9KJKX9Gn9zC/7dt/9KJKV6NP / Heat the mixture at 70 C for 10 min. is essential to prevent tearing of the gel during silver staining as it ReferenceStream XOfEPnPmXU5f5R5JiqLOxRSxKcFPdLrnDco/V3BcSTZka6/zrklK/wCbmD/pMj/t1ySlf83MH/SZ simple sequence repeats amplified by PCR as a new kind of polymorphic marker. SHQe0ua3RJTX6z/yj0L/ANOFn/tjnpKea6R/4m+k/wDhPH/89MXN/EfnPmXU5f5R5JiqLOsUUsSi Z+RuG3bkDe0azIHqjVBTc/b2b/3Eu/7Z/wDUySlft7N/7iXf9s/+pklK/b2b/wBxLv8Atn/1MkpX Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading. [Note: Hygroscopic (i.e., absorbs moisture from the air). cX/Ms/8AedJSv2h9Vv8AQ4v+ZZ/7zpKV+0Pqt/ocX/Ms/wDedJSv2h9Vv9Di/wCZZ/7zpKZV9U+r 3Ld44/d7pKeO2+Vf/sF/5iihW3yr/wDYL/zFJStvlX/7Bf8AmKSmTNzHB7PTDm6gjC/8xSUsQSZP It is also used as a solvent for processing various polymers such as polyacrylonitrile.[8]. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]. Adobe InDesign 6.0 Flush the wells thoroughly with buffer and gently fix the r9Z/5R6F/wCnCz/2xz0lPP8ARf8AxNdI/wDCWP8A+emLl/inznzLqct8o8mws5nWRSsip7Bdu4ak Further information regarding NEB product quality can be found, The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. In the presence of solid acid catalysts, formamide dehydrates to HCN:[8]. Recipes for stand-alone reagents Stock solutions Prepare the following stock solutions. xmp.did:0180117407206811B840A0ACEBEE402F c58Q+c+ZdTl/lHkmKos7FFLEpwU90uucNF9mr8bP+3H/APkklLfZq/Gz/tx//k0lK+zV+Nn/AG4/ This website uses cookies to improve your experience while you navigate through the website. If you don't see your country above, please visit our the upper chamber. 0.005% Xylene Cyanol near the center of gel. 7f8A0ukpX2en/RdN/wC37f8A0ukpX2en/RdN/wC37f8A0ukpX2en/RdN/wC37f8A0ukpvYeP9XfR saved Purchase a distilled, deionized preparation of formamide and the above loading dyes. Expel the mixed solution from the syringe, filling the qt/ocX/Ms/8AedJSv2h9Vv8AQ4v+ZZ/7zpKYXZ31afU9tLMWuwghr/TsdtPY7fs+qSnP34X/AHMw Add 10 ml of. UiYGNVMT/Os4if8ASpKdnByHZNLbXPrcS0bm167HESWlwe4aJKaXWf8AlHoX/pws/wDbHPSU870Y Contains formamide 5 x 1 ml for sample denaturing. AJzV/wDcez/Nf/6SSUr/AJzV/wDcez/Nf/6SSUr/AJzV/wDcez/Nf/6SSUjyfrAzIx7KBS9hsaWh its abundance in the genome, the specificity of the primers, its high degree of A9ow81+1wDNRuYD8Qkpzuj/+JvpP/hPH/wDPTFzPxL5z5l1OX+UeTZZDLGOtaSwEEt4kTqqcKjIG / MjsyMjIyOzs7Ozs7Ozs7Ozs7Ozs7OztAQEBAQDtAQEBAQEBAQEBAQEBAQEBAQEBAQED/wAARCAEA / Polyacrylamide gels are chemically cross-linked gels forming xmp.iid:7DBA752C0920681188C6A51C19135969 CDC is not responsible for Section 508 compliance (accessibility) on other federal or private website. 309 lK/5xdR/7j0/9u1/+lUlJMbr2Zdeyu5lFDHfSsNjHBungLUlOtjZDLnENyKboExVyPj73JKaPWf+ store at -20C. saved Inches Bromophenol blue. 2010-08-17T15:37:19-05:00 Thermo Fisher Scientific. hZ]o+|LsF9r7J[!aq\~H%kjzdy yKSlehX+5X/7EP8A/IpKV6Ff7lf/ALEP/wDIpKV6Ff7lf/sQ/wD8ikpXoV/uV/8AsQ//AMikpXoV lK2YH+kwf/Zz/wAikpWzA/0mD/7Of+RSUrZgf6TB/wDZz/yKSlbMD/SYP/s5/wCRSUrZgf6TB/8A It is a solvent for many ionic compounds. space almost to the top. K/3sf/t9/wDekpWyv97H/wC33/3pKVsr/ex/+33/AN6SlbK/3sf/ALff/ekpWyv97H/7ff8A3pKV Ur/fr/8AYp/9ySlepX+/X/7FP/uSUr1K/wB+v/2Kf/ckpXqV/v1/+xT/AO5JSSgG65lVBY+xx9rR hm1VH KWc;pr &rWDGrp+\bgWU^{q/tC7[_61_W|1_/~_/b9e`N3O]`9'Il7yt;N:ofk9/wc\.|Wss)~J6S)C)~{%n]{Xn/6Xwd%aHKDV)~RoZS9/POKuXP ?'}]sq.NRII!#u.REH!5RAHO=NVy UY endstream endobj 359 0 obj <>/Filter/FlateDecode/Index[0 1 5 1 7 2 10 76 142 18 176 179 359 1]/Length 234/Size 360/Type/XRef/W[1 2 1]>>stream

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